ABSTRACT
The aim of study is to establish pharmaceutical equivalence of different brands of Metformin tablets available in Karachi, Pakistan. The quality control parameters which are studied are weight variation test, hardness test, thickness, friability, disintegration and dissolution specified by BP/USP [British and United State Pharmacopoeia]. Weight variation and hardness value requirement was complied by all brands. Disintegration time for all brands was within range i.e. 15 minutes and also complies with the BP/USP recommendation. All brands showed more than 90% drug release within forty five minutes. The present conclusion suggests that almost all the brands of Metformin that are available in Karachi meet the specification for quality control analysis. Assay performed by HPLC by keeping flow rate of 1.0 ml/min of the mobile phase and the quantitative evaluation at 225 nm was performed. The retention time of Metformin was found to be 2.5min. Method suitability for the quantitative determination of the drugs was proved by validation according to the International Conference on Harmonization [ICH] guidelines
ABSTRACT
Sensitive, simple, reliable and rapid HPLC technique for the estimation of simvastatin [SMV] and cetirizine has been designed in this study. The chromatographic conditions were set using Shimadzu LC-10 AT VP pump, with UV detector [SPD-10 AV-VP]. System integration was performed with CBM-102 [Bus Module]. Partitioning of components was attained with pre-packed C-18 column of Purospher Star [5 microm, 250 x 4.6 mm] at ambient conditions. Injected volume of sample was 10 microl. Mobile phase was composed of 50:50 v/v ratio of Acetonitrile/water [pH 3.0 adjusted with ortho-phosphoric acid] having 2 ml/minutes rate of flow. Compounds were detected in UV region at 225 nm. Percent Recovery of simvastatin was observed in the range of 98-102%. All results were found in accept table range of specification. The projected method is consistent, specific, precise, and rapid, that can be employed to quantitate the SMV along with cetirizine HCl. It was estimated by 3 successive cycles of freeze and thaw stability. Results of FT samples were found within accept table limits the method was developed and validated in raw materials, bulk formulations and final drug products
ABSTRACT
Plasmodium falciparum is the most well-known reason for extreme and life-debilitating malaria. Falciparum malaria causes more than 1 million deaths annually. Malaria remains a noteworthy reason for major morbidity and mortality in the tropics, with Plasmodium falciparum accountable for the mainstream of the disease weight and Plasmodium vivax being the geologically greatest broadly dispersed cause of malaria. The controlling of severe malaria comprises quick direction of suitable parenteral anti-malarial agents and initial acknowledgement and treatment of the complications. This clinical trial was piloted in 100 patients, in which 50 received the test drug [Malarina] and 50 received the control drug [Quinine Bisulphate]. The age range of patients was 12 years to above 50 years. The sample paired t-test was applied to evaluate the significant level. Malarina was very effective in treating malaria sign and symptoms. The new treatment Malarina was safe and well tolerated in all patients
ABSTRACT
The results of pollen morphological studies [LM] of 9 Pakistani Rosa species [R. beggeriana, R. brunonii, R. ecae, R. foetida, R. lacerans, R. macrophylla, R.. multiflora, R. nanothamnus and R. webbiana] have been presented in this paper. All examined pollen grains. are small, heteropolar, and tricolporate. A11 grains except R. foetida tend to have very long colpi. Constricted equatorial bridges make the Pori invisible in most of the species. The sculpture of grain confirms the striate pattern. The palynological features of exine thickness, shape, equatorial and polar diameter of pollen grains and length of colpi are found useful criterion for species delimitation while sculpturing and size at LM turned out to be a poor criterion. A dichotomous key is constructed for the species identification using the characters that has been studied